[
    {
        "id": "authors:wp1g5-a0413",
        "collection": "authors",
        "collection_id": "wp1g5-a0413",
        "cite_using_url": "https://authors.library.caltech.edu/records/wp1g5-a0413",
        "type": "article",
        "title": "Basement membrane perforations guide anterior\u2013posterior axis formation",
        "author": [
            {
                "family_name": "Chen",
                "given_name": "Dong-Yuan",
                "orcid": "0000-0003-2179-2847"
            },
            {
                "family_name": "Claussen",
                "given_name": "Nikolas H.",
                "orcid": "0000-0002-9020-6437"
            },
            {
                "family_name": "Titus",
                "given_name": "Shiny"
            },
            {
                "family_name": "Hu",
                "given_name": "Wenqi",
                "clpid": "Hu-Wenqi"
            },
            {
                "family_name": "Weatherbee",
                "given_name": "Bailey A. T.",
                "orcid": "0000-0002-6825-6278"
            },
            {
                "family_name": "Mandelbaum",
                "given_name": "Rachel S.",
                "orcid": "0000-0001-8611-1462"
            },
            {
                "family_name": "Scott Jr",
                "given_name": "Richard T."
            },
            {
                "family_name": "Seli",
                "given_name": "Emre"
            },
            {
                "family_name": "Streichan",
                "given_name": "Sebastian J.",
                "orcid": "0000-0002-6105-9087"
            },
            {
                "family_name": "Zernicka-Goetz",
                "given_name": "Magdalena",
                "orcid": "0000-0002-7004-2471",
                "clpid": "Zernicka-Goetz-M"
            }
        ],
        "abstract": "Establishment of the anterior-posterior (AP) axis is a critical symmetry-breaking event in mammalian development. In mice, this process involves the directed migration of the distal visceral endoderm (DVE). Here, we use targeted perturbations to demonstrate that asymmetric perforations in the basement membrane guide DVE migration. During implantation, matrix metalloproteinases in extra-embryonic tissues create uneven basement membrane perforations, establishing directional cues for cohesive DVE migration. Using light-sheet microscopy and tissue cartography, we show that migrating DVE deforms surrounding tissues. Physical modeling and live imaging of DVE protrusions indicate that basement membrane perforations orchestrate active force generation within the DVE. Extending these findings to human embryos and stem cell-derived models, we identify basement membranes with enriched perforations near the anterior hypoblast in embryos, suggesting a conserved mechanism for AP axis specification. These findings reveal an unrecognized role of basement membrane remodeling and mechanical heterogeneity in guiding directional tissue migration during mammalian development.",
        "doi": "10.1038/s41467-025-61441-6",
        "pmcid": "PMC12284222",
        "issn": "2041-1723",
        "publisher": "Nature Publishing Group",
        "publication": "Nature Communications",
        "publication_date": "2025-07-22",
        "series_number": "1",
        "volume": "16",
        "issue": "1",
        "pages": "6763"
    },
    {
        "id": "authors:rryjh-r3641",
        "collection": "authors",
        "collection_id": "rryjh-r3641",
        "cite_using_url": "https://authors.library.caltech.edu/records/rryjh-r3641",
        "type": "article",
        "title": "Atlas of amnion development during the first trimester of human pregnancy",
        "author": [
            {
                "family_name": "Hu",
                "given_name": "Wenqi",
                "orcid": "0000-0002-7934-9429",
                "clpid": "Hu-Wenqi"
            },
            {
                "family_name": "Sancho-Serra",
                "given_name": "Carmen"
            },
            {
                "family_name": "Gantner",
                "given_name": "Carlos W.",
                "orcid": "0000-0003-0825-7786"
            },
            {
                "family_name": "Szafranska",
                "given_name": "Hanna M.",
                "clpid": "Szafranska-Hanna-M"
            },
            {
                "family_name": "Solanky",
                "given_name": "Nita",
                "orcid": "0000-0001-8304-5543"
            },
            {
                "family_name": "Metcalfe",
                "given_name": "Kate"
            },
            {
                "family_name": "Vento-Tormo",
                "given_name": "Roser"
            },
            {
                "family_name": "Zernicka-Goetz",
                "given_name": "Magdalena",
                "orcid": "0000-0002-7004-2471",
                "clpid": "Zernicka-Goetz-M"
            }
        ],
        "abstract": "<p>The amnion is a critical extra-embryonic structure that supports foetal development, yet its ontogeny remains poorly defined. Here, using single-cell transcriptomics, we identified major cell types and subtypes in the human amnion across the first trimester of pregnancy, broadly categorized into epithelial, mesenchymal and macrophage lineages. We uncovered epithelial&ndash;mesenchymal and epithelial&ndash;immune transitions, highlighting dynamic remodelling during early pregnancy. Our results further revealed key intercellular communication pathways, including BMP4 signalling from mesenchymal to epithelial cells and TGF-&beta; signalling from macrophages to mesenchymal cells, suggesting coordinated interactions that drive amnion morphogenesis. In addition, integrative comparisons across humans, non-human primates and in vitro stem cell-based models reveal that stem cell-based models recapitulate various stages of amnion development, emphasizing the need for careful selection of model systems to accurately recapitulate in vivo amnion formation. Collectively, our findings provide a detailed view of amnion cellular composition and interactions, advancing our understanding of its developmental role and regenerative potential.</p>",
        "doi": "10.1038/s41556-025-01696-9",
        "issn": "1465-7392",
        "publisher": "Nature Publishing Group",
        "publication": "Nature Cell Biology",
        "publication_date": "2025-07-14",
        "volume": "27",
        "pages": "1175\u20131185"
    }
]